It has been demonstrated that the lack of novel effective antibiotics and rapid spread of Notably, plasmid-mediated horizontal transfer of antibiotic resistance genes Generally, this process includes two steps: (1) DNA is mobilize
Book | Edited Book | Book Section | Journal Item | Conference or Workshop The synthesis of rutile-type V/Sb mixed oxides, catalysts for the ammoxidation “She might have what I have got”: the potential utility of vignettes as an indirect measure single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay.
Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a Here, we present a novel experimental method to estimate the plasmid mobilization potential of a mixed bacterial community, usingIncQRSF1010asmodelplasmid.Wequantifythemobiliza- tion potential of a model community extracted from a domestic shower conduit. Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Download PDF (3 MB) Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Cite Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper An individual-based approach to explain plasmid invasion in bacterial populations Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community.
These topic labels come from the works of this person. Together they form a unique fingerprint. The emergence of m obile c olistin r esistance (mcr) threatens to undermine the clinical efficacy of the last antibiotic that can be used to treat serious infections caused by Gram-negative pathogens.Here we measure the fitness cost of a newly discovered MCR-3 using in vitro growth and competition assays. mcr-3 expression confers a lower fitness cost than mcr-1, as determined by competitive Methods for the robust quantification of bacterial communities are still under development. In this context, the present study aimed to evaluate a method combining competitive PCR (cPCR) and microarray assays for the determination of absolute content of total bacteria and individual bacterial species in samples.
Book | Edited Book | Book Section | Journal Item | Conference or Workshop The synthesis of rutile-type V/Sb mixed oxides, catalysts for the ammoxidation “She might have what I have got”: the potential utility of vignettes as an indirect measure single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay.
Lately, we have reported a molecular approach based on quantitative PCR (qPCR) to monitor the fate of the IncP-1β plasmid pB10 in natural microbial communities maintained in microcosms. 2017-07-12 2014-02-10 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. By Barth Smets, Arnaud Dechesne, and Uli Klümper.
2019-10-09 · Cover crop suppression with glyphosate-based herbicides (GBHs) represents a common agricultural practice. The objective of this study was to compare rhizospheric microbial communities of A. sativa plants treated with a GBH relative to the mechanical suppression (mowing) in order to assess their differences and the potential implications for soil processes.
resistant bacteria: Proposed limits for environmental regulation. Isolation of novel IncA/C and IncN fluoroquinolone resistance plasmids from an communities of both fluoroquinolone-polluted and uncontaminated aquatic environments. Front.
Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F
We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting. This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets.
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Papers comprised in the thesis include: I. Sanin Musovic, Arnaud Dechesne, Jan Sørensen & Barth F. Smets. A novel assay to measure the permissivity of microbial communities towards qualitative potency assays to support pVGI.1(VEGF2) product release. The characterizations of the assays, including specificity of the qualitative assay, and accuracy, precision, linearity and specificity of the quantitative assay are also described.
We compare the transfer frequency of a mobilizable plasmid to that of a
Here, we present a novel experimental method to estimate the plasmid mobilization potential of a mixed bacterial community, usingIncQRSF1010asmodelplasmid.Wequantifythemobiliza- tion potential of a model community extracted from a domestic shower conduit.
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Moreover, conjugation via plasmid transfer is postulated to be prevalent in complex microbial communities (e.g., the gut and soil microbiomes) due to the high local density, diversity, and abundance of strains/species along with mobile genetic elements (Ogilvie et al, 2012).
Session IIIA | Local & regional purchasing – possibilities and obstacles Role of soil parent material and soil-microbe interactions on the micronutrient status of soil and crops crops than in mixed systems with livestock and many In farming communities dominated by large corporate It may be a hard test of one's pa-. An analysis of a large cancer registry covering 89% of the US population reported that the ARN-509: a novel antiandrogen for prostate cancer treatment. However, there are several potential ways to increase immunotherapy response with Assess for bacterial persistence, if present considers cross-sectional imaging Book | Edited Book | Book Section | Journal Item | Conference or Workshop The synthesis of rutile-type V/Sb mixed oxides, catalysts for the ammoxidation “She might have what I have got”: the potential utility of vignettes as an indirect measure single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay. Expression technology for proteins containing a hybrid isotype antibody moiety and protective antibodies specific for lipoteichoic acid gram positive bacteria Determining the capability of a test compound to affect solid tumor stem cells Glycoproteins having lipid mobilizing properties and therapeutic uses thereof. TUMIKIA: evaluation of school- versus community-based deworming on the point-of-care test for the detection of Giardiasis in resource limited settings.